Sentence examples for labeled pooled from inspiring English sources

The comparative two-dimensional gel analysis was performed using an established experimental design [34] in which the high variation between gels is minimized by including a common, labeled pooled sample in all gels.

This process allows each investigator's samples to be individually labeled, pooled with other samples, and automatically separated after bulk sequencing.

In a typical array CGH experiment, DNA samples being compared (e.g. disease versus control) are differentially fluorescently labeled, pooled and hybridized to oligo probes spanning the genome of interest printed on a glass slide.

Briefly, reference genomic DNA and test DNA are differentially labeled, pooled, and hybridized onto arrays comprising BAC, cDNA, or oligonucleotides, and imbalances are visualized as differences in fluorescence intensity.

The labeled, pooled and denatured (3 minutes at 95°C) samples in a hybridization mixture containing 25 μg mouse Cot-1 DNA (Invitrogen), 40 μg poly- dA) DNA (Opoly- dA5% formamiDNA(Sigma), 5xSSC and 0.1% SDS were applied under a LifterSlip cOperonErie Scientific Company,25%, USA) and hybridized formamide hourSigma42°C in a water bath.

Peptides were iTRAQ labelled, pooled and separated by immobilized pH gradient isoelectric focusing (IPG-IEF) on narrow range pH 3.7 4.9 and 4.00 4.25 strips, as described previously (21).

Overgo probes were radioactively labelled, pooled and hybridised to tammar wallaby (Me_KBa; Arizona Genomics Institute) BAC library filters as previously described [ 11].

Magnetic beads bound to anti-FITC antibody were reacted with the labeled pool of chromosomes and separated by exposure to a magnetic field.

To quantify how the increased temperature treatment influenced the flow of recently fixed C and leucine-derived N, we used the bulk PP and BP rates, the median stable isotope incorporation values, and the cell abundances to calculate the total incorporation for each of the two labeled pools (Popa et al., 2007; Adam et al., 2016; Supplementary Table S1,Table 1).

Further information is also provided on the distribution and size of differentially labeled pools of AChRs (Fig. 6).

The labeled pool was eluted twice in 20 ul of preheated water each and dye incorporation was quantified using a Nanodrop.