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Each 10 μL PCR reaction included 1ul extracted DNA, 5 μL Qiagen master mix (Qiagen Type-It Microsatellite Kit, Qiagen Inc ., 0.2 μL of each reverse primer, 0.2 μL (dye-labeled) or 0.1 μL (3-primer labeled) of each forward primer, 0.15 μL FAM-labeled 3-primer tag for each 3-primer-labeled primer pair, and water to total 10 μL.
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After labelling of each amplified RNA with Cy3 and Cy5, the reactions were purified with the LiCl method and used in hybridisation experiments.
Separate analyses were completed for VOC and PCB quantification, each requiring 200 μL of serum spiked with H labeled versions of each VOC or C labeled versions of each PCB.
Finally, for each cDNA sample the Cy3 reference labeled fractions of each sample were combined and mixed separately with each Cy5-labeled cDNA fractions at a 1 1 ratio.
The t-test is performed by using the relative intensities of the isotopologues in the labeled samples of each condition.
The labeling tag of each construct was covalently modified with AF555 by the Sfp enzyme using a published protocol.
In class comparison analysis, the data set consists of labeled specimens and each of them has a predefined class assignment.
Even though dye labeling of each protein occurred in an essentially 1 1 ratio, slight differences in labeling efficiency were noted, with the labeling of hPPARα (1.0 1) being slightly more efficient than the labeling of hLXRα (0.93:1).
He began reading the label of each and every drug.
The anatomical label of each ROI was determined based on the locations of peak coordinates of the clusters.
The classifier is trained to determine the phoneme label of each frame based on the observed EMA features.
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