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Immunopositive dotty elements and thin processes took up the neuropil of the labeled layers where they intermingled with mGluR5 immunoreactive somatodendritic domains (Fig 1A, A').
Based on our experience, electroporation of the cortical neural progenitors at E16 labeled layers 2/3 cells.
The cross-sectional OCT structural image (Fig. 1A ) shows a typical anatomy of the rat eye with labeled layers and optic nerve head (ONH) area.
Each fluorescent image was merged to measure the distance between fluorescent labeled layers at the interface between native bone and the implanted scaffold (INBS) and at the central pore area with the Nikon NIS Elements software (Nikon Instruments Inc., Melville, NY, USA) tools.
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Many labeled layer V CCS pyramidal cells are seen.
Representative examples of axonal projection patterns from labeled layer 2/3 neurons in infragranular sections are shown in Figure 7.
Coronal sections showed that labeled layer 2/3 neurons had dense axonal projections in infragranular layers in addition to their projections in supragranular layers.
Formation of an axonal plexus in layer V by labeled layer II/III neurons is observed at P10, and dense layer V innervation is seen at P14.
The labeled layer 2/3 pyramids invariably had dense projections in supragranular layers extending in all directions from the injected D2 column into surrounding columns.
The D2 barrel was difficult to accurately reconstruct in several animals as a consequence of the dense descending axonal projection passing through layer 4 from the labeled layer 2/3 neurons.
These results suggest that the infragranular projection of the labeled layer 2/3 neurons was not altered and that the axonal arborizations of these cells can be modified by experience-dependent mechanisms in a layer-specific manner.
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