Labelling in whole embryo culture showed that nucleotides are taken up by the neurulating embryo and incorporated into genomic DNA.
The combined effects were synergistic for labeling in the bronchioles but approximately additive for labeling in whole-lung parenchyma.
There are about 150 cells labeled in the whole larva.
To visualize CTB-594 labeled axons in whole ONs, mice were then killed 48 h post injection and ONs cleared using a previously published protocol.
Transcripts of HuR and ADAR1 targets were synthesized and biotin labeled in vitro, and incubated with whole-cell lysates.
Recently, azF in CCR5 was chemically ligated to triarylphosphine-conjugated FLAG peptides to label GPCRs in whole cells.
In the present study, we, therefore, used a three-dimensional (3D) reconstruction of fluorescently labeled axons in whole-mounted, cleared optic nerves to determine the pattern of axonal regeneration, similarly to what has been done in the injured spinal cord.
Yellow dashed lines indicate the location of the whole coelomic pouch with the ectopically placed micromeres labeled in green.
We also observed dotted localization of labeled dsRNA actin in whole adult female ticks (Fig. 1C).
Long- and very-long-chain fatty acids are hydrophobic and therefore pose substantial delivery issues when performing labeling studies in whole animals.
