Within the rolls, that certainly recall Egyptian papyrus scrolls, are her prints and drawings, now forever sealed and labeled in dark waxes, calling us to wonder what buried images and imagination rest closed inside.
The transmigrated cells were labeled in dark blue color after migrating from the loaded upper compartment of chamber to the lower compartment with or without adding DFO for studying the cell mobility in normoxia (vehicle) and hypoxia (DFO)).
The transition from monostable to bistable is known to be governed by a supracritical pitchfork, the transition from bistable to tristable involves a subcritical pitchfork bifurcation, and the transition from tristable to monostable takes place through two simultaneous saddle-node bifurcations involving the two attractors labelled in darker blue in Figure 2C.
In brief, cells were rinsed in PBS supplemented with 0.1 mM CaCl2 and 1 mM MgCl2 (PBS++), labeled in the dark on ice for 20 minutes with 1 mg/mL EZ-link Sulfo-NHS-LC-biotin (Pierce) in a solution of 10 mM triethanolamine, pH 8, 2 mM CaCl2, and 150 mM NaCl.
Interestingly, a total of 182 (25%) proteins with unknown functions were differentially labeled in between the light-dark cycle suggesting their potential roles in cellular metabolism, and highlight the importance of systematic characterization of their functions for better understanding of the physiology and cellular metabolism of Cyanothece.
Five gene coexpression modules, labeled by blue, dark gray, dark orange, light yellow, and yellow, were found to be significantly enriched with genes that were specifically duplicated in A. thaliana (fig. 2 B).
Importantly, the most strongly contacted cells in this group (labeled with darker reds in Figure 5H) have relatively high neurogranin expression.
The brilliant descriptions in these tapestries of exotic plants and animals, local customs, and human endeavor document a thriving arts scene in an era often labeled the "Dark Ages".
I feel I can say this because I was born and raised in Karachi, the city Time Magazine famously labeled "Pakistan's Dark Heart" in 2012.
In the one section of each 4, reacted for CTB-gold, labeled cells were identified in dark field by the granular precipitate in the cytoplasm.
We conducted 2D-DIGE as follows: The IPG strips (24 cm, pH 3 10, and NL) were rehydrated with labeled samples in the dark overnight with rehydration buffer (7 M urea, 4% w/v CHAPS, 20 mM dithiothreitol (DTT), and 1% v/v IPG buffer with a trace amount of bromophenol blue).
