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In the Chicago study, four-pound defibrillators, which cost about $2,500 each, were distributed like fire extinguishers in labeled glass cabinets at O'Hare, Midway and a third airport, Meigs Field.
The membranes were placed on labeled glass slides with migrated cells facing up.
Cellulose samples (1‰ w/w) were suspended in deionized water on labeled glass microscope slides, then digital photomicrographs were taken using a stereomicroscope (SMZ 1500; Nikon, Tokyo, Japan).
The samples were allowed to warm to room temperature and were pipetted into labeled glass test tubes in 1.0 ml aliquots, to which 100 μ l dextran blue B-20000, 2 000 000 Da; Sigma Chemical Co, St Louis, Missouri, USA) was added.
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"You know what this reminds me of, is Leonardo da Vinci," said Mary Bandziukas, 50, admiring a drawing of an invention labeled "glass-blowing apparatus".
Cells were given three final washes in PBS followed by two washes in dH2O and then mounted onto labelled glass slides.
After the 2-week period, samples were removed from the saline solution using pre-cleaned tweezers and placed on labeled microscopic glass slides under a fume hood until the samples were dry.
These preparations were then stored in dried, labeled brown glass bottles and stored in desiccators for further process.
These mixtures were passed through a sieve of 180 μm mesh size, placed in dried, labeled brown glass bottles and then kept in desiccators at room temperature for further analysis.
As illustrated in Equation 1 data obtained from slides with spot-localized contaminating fluorescence will report green channel intensities that are falsely high (Gtotal is the total signal acquired in the green channel; GCy3, Gglass, and Gcont are the signals arising from the Cy3 labeled DNA, glass substrate and contaminant, respectively).
Climbing upwards, there is absolutely no contest between a supermarket own-label glass of champagne and the fragrant joy of this pinot noir: St Aubin 1er Cru 2006 (£17.99, Marks & Spencer; 13.5% abv) is just beginning to develop, so that you catch a hint of autumn leaves and mushrooms on the nose.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com