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[18F]PBR06, a fluorine-18 labeled form of PBR06, is a promising PET TSPO radioligand originally developed at NIMH. [11C]PBR06, a carbon-11 labeled form of PBR06, was designed and synthesized for the first time.
The bound molecules are detected on autoradiographs by the failure of binding of a radio labeled form of the peptide under study; the probes are of the same dimensions as glass microelectrodes and hence are relatively atraumatic.
To find these patterns, researchers used to expose rats to an odor for 45 minutes--an unnaturally long time--then kill them and look for changes in the uptake by the olfactory bulb of a labeled form of glucose, which indicates neuronal activity.
To investigate gastrointestinal absorption of intact gluten peptides, we dosed a gluten-sensitive macaque with an isotopically labeled form of the immunotoxic 33-mer gluten peptide [13], and measured its plasma concentration using a sensitive and specific mass spectrometric method.
One of these mutants has been used to prepare a fluorescently labeled form of CNTF, a therapeutically important protein.
The latter compound was used to create a fluorescently labeled form of Ciliary Neurotrophic Factor (CNTF), a protein of therapeutic importance.
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Unlabeled, 15N-labeled and 13C,15N labeled forms of the protein were used for different NMR experiments.
Consequently, numerous assays have been developed to study such systems that involve a variety of derivatives and isotopically labeled forms of these cofactors.
For instance, the iTRAQ 4-plex labeled forms of peptides typically eluted before the iTRAQ-8-plex labeled forms of the same peptides (see also below).
BaF/3 cells were stably transfected with fluorescently labeled forms of Grb2 (Grb2–mRFP) and EGFR (EGFR eGFP).
To date imaging studies in animal models have been carried out predominantly with various labeled forms of annexin A5.
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