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Fresh or expanded cells were CFSE labeled, extensively washed in sterile saline, and concentrated in 200 µl of sterile PBS in preparation for tail-vein injection to each mouse.
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Basal dendrites arborizing mainly in the same layer around the cell body were also labelled extensively.
Using confocal microscopy, it was found that the fluorescently labeled nanoparticles extensively accumulated in the cytoplasm and the nucleus of cancer cell lines used for the study (Jain et al. 1998).
2 3 In addition, bevacizumab, a monoclonal antibody related to ranibizumab that also binds all VEGF isoforms, has been used off-label extensively to treat a variety of ocular neovascular conditions.
The show proceeds in more than two dozen large, densely installed, extensively labeled vitrines that resemble department store displays and also little cities, given the buildinglike Art Deco forms of many of the bottles.
"When you go to the grocery store," Aral said, "the food is extensively labeled.
The oxidation behavior of the resulting 11 methionines can be grouped into three categories: (1) extensively labeled both in native BR and in SDS (loop residues M32, M68, and M163); (2) protected in the native state but not in SDS (M20, M118); (3) always protected (M56, M60, M93, M145, M179, M209).
Metabolically labeled cells were extensively washed in a 10-cm dish with isotonic buffer.
The labeled RNA was extensively washed in 70% ethanol, dried and resuspended in 16 μl water.
Experiments in which labeled carriers were extensively washed with buffer confirmed this notion, showing that wash-out of either luminophore was not detectable.
In contrast to oxidative tissues such as heart and muscle, where under most conditions, specific acetyl-CoA C-isotopomers can be related to the catabolism of specific C-enriched oxidative precursor substrates [ 10, 11], hepatic acetyl-CoA is also extensively labeled from Krebs cycle intermediates via pyruvate recycling.
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