Newton et al. labelled embryonic chondrocytes and found that some develop into resting-zone stem cells.
Techniques are described for labeling embryonic chick motoneurons in vivo with Lucifer Yellow or fluorescein isothiocyanate conjugates of wheat germ agglutinin (Fl-WGA).
On the other hand, mosaic analysis with a repressible cell marker (MARCM), which was used to uncover secondary lineages, does not label embryonic lineages as the perdurance of Gal80 prevents their visualisation (Lee and Luo, 1999).
When we labelled embryonic skin for markers of undifferentiated epidermis, we found that some areas in E14.5 embryos still had a single layer of epithelial cells, which was largely lost at E15.5.
By injecting high concentrations of mRNA encoding H2B-mCherry, we labeled the embryonic beta-cells with nearly uniform levels of H2B-mCherry at 3 dpf (Fig. 5a).
In the afterglow of last week's summit meeting, the rescue fund, known as the E.F.S.F., was quickly labeled an embryonic European monetary fund.
To do so, we genetically labeled the embryonic beta-cells with GFP at 3 dpf using Tg ins:CRE-ER T2 ) and the CRE-reporter line, Tg ins:Red-Stop-Green), and traced them until 30 dpf (Fig. 6a).
Experiments observing the steady-state pattern of chimeric labeled mouse embryonic stem cells [36], and pulse-lineage-labeled newborn Clara cells [17] show that for homeostasis of the intrapulmonary respiratory epithelium, most progenitor cells (e.g. Clara cells) are randomly distributed throughout the bronchioles and are not associated with particular anatomical niches.
Tissue samples were individually labeled and embryonic tails were used for genotyping by PCR.
In an attempt to test whether there is loss of neurons over time, LM112 animals were staged and the number of cholinergic neurons in the ventral nerve cord was counted using the marker unc-17::GFP that labels embryonic and postembryonic motor neurons (21, 22).
