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While the software scans your photo collection for familiar faces and suggests name tags for people you have labeled during the setup process, you still may have to verify the suggested tags with a few mouse clicks.
For viability and cell cycle analysis, cells were labeled during the last hour of culture with the DNA dye Hoechst 33342 (SIGMA) (5 µg/ml, 60 minutes in incubator at 37°C with 5% CO2).
DOP-PCR-amplified chromosome-specific DNA was labeled during the secondary PCR by incorporating biotin-16-dUTP (Roche, Switzerland) or Cy3-dUTP (GE Healthcare, UK) [ 48].
Ameloblasts were heavily labeled during the transition and maturation phases of amelogenesis, but labeling was absent from ameloblasts during the secretion phase.
It is noteworthy that numerous ER-α-negative and PR-negative cells were also labeled during the 5-day labeling period.
The mRNA samples corresponding to treatment (infested) and control (non-infested) was labeled during the cDNA synthesis with Cy3- or Cy5- labeled dUTP and with one technical replicate labeled by reversed dye compared to the first hybridization.
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In contrast, the pools of both G6P and sucrose were rapidly labelled during the pulse and de-labelled during the chase.
From the responses provided by the participants several sub-themes were labelled during the coding of the transcripts.
If this is the case, the total number of cells labelled during the BrdUrd pulse remains constant throughout the cycle, provided that cells produced after division are excluded.
Due to the rapid first-pass extraction of 99mTc-HMPAO [ 29], brain images reflect the average activity that was labelled during the third and fourth blocks.
In these transgenic embryos, the dorsal midbrain is sparsely but strongly labelled during the first few days of development (Fig. 3A).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com