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The first was labeled directly with Hoechst 33342 (FRESH), and the others were processed as smears.
Non-adherent cells can be labeled directly in suspension in the medium.
The first was labeled directly with Hoechst 33342, and the others were processed as smears.
A specially designed oligonucleotide probe labeled directly by alkaline phosphatase (AP) and highly sensitive chemiluminescent substrate CDP-star for AP was used in the hybridization assay.
Briefly, BAC clone DNA probes were labeled directly with Spectrum Orange-dUTP or Spectrum Green-dUTP using a commercially available kit (Abbot Molecular/Vysis).
To further confirm the differences in edeine sensitivity between ribosomes bound to either the AUG or CUG start codons, we used mRNA labeled directly with [α35S]-CTP (Fig. 5D, left panel).
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The targets labelled most intensively were produced by the Cy3-primer labelling (2 of 3 viruses) or by the indirect labelling method (1 of 3 viruses), the weakest signal showed targets labelled directly (all 3 viruses).
Prohoroff et al. (2012) proposed that clastic sedimentary rocks depositionally overlie the larger bodies in Marin County ("sfs" label directly above "Marin Headlands vfr" label on Fig. 2), but the contact relationships are disputed (Raymond and Bero 2015).
DNA probes were labelled directly by NICK translation with SpectrumOrange-dUTP or SpectrumGreen-dUTP (ABBOTT, Illinois, USA).
The samples and a reference RNA Universal Human Reference RNAA, Stratagene, La Jolla, CA) were labelled directly with Cy3-dCTP (reference RNA) or Cy5-dCTP (sample RNA) (Amersham Biosciences, Little Chalfont, UK) and indirectly with 3DNA Submicro Expression Array Detection kit (Genisphere, Montvala, NJ).
Probes for the newly identified transposable elements were labelled directly with Texas red (Invitrogen, Camarillo, CA, USA) using Nick translation approach [ 69] of PCR product from primers designed for insertion site and internal regions of the transposons.
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