In sequences of consecutive horizontal sections (0.5 µm), individual cones were either labeled by JH455 (specific to SWS1 or S-cones, gray arrowheads) or by JH492 (specific to M/LWS or M-cones, white arrowheads).
In this experiment, Kv2.1 α-subunit was transected into HEK-293 with or without overexpressing flotillin-1 and then labeled by a specific Kv2.1 antibody.
On tissue sections, melanocytes at the basal layer of healthy skin were labeled by a specific nuclear signal using a rabbit PAX3 antibody or a mouse MITF antibody.
> -wrap-foot> focalocorticalical dysplasia; immunoreactivity: − not present, + moderate, ++ strong In each slice, we assessed the number of neurons and astrocytes labeled by a specific Ab on the total number of each cell type within the lesion using an ocular grid [ 4].
Cells with EdU incorporation were labeled by an EdU specific antibody conjugated to an A647 florescent marker.
The cDNA was amplified and labeled by using gene specific oligonucleotide primers of target genes and SYBR® PremixExTaq II kit (TaKaRa Bio Inc. Shiga, Japan).
DNA probes were internally labeled by synthesis with specific dsDNA templates, sense and antisense primers and Pfx DNA polymerase (Invitrogen) in the presence of [α-P]dCTP.
In the GL and OL of human MR muscle the fast or type II fibers were labeled by the antibodies specific to rat MyHC-2a (SC-71), -2x (BF-35, stains all MyHC except -2x, not shown, see our previous study [ 51]), and weakly with the antibody BF-F3, against rat MyHC-2b (Fig. 3).
HSV-1 0−GFP encoded a truncated ∼55 kDa protein that was only labeled by the GFP-specific antibody, whereas the 0+GFP viruses encoded ∼140 kDa ICP0+GFP-12, -24, and -105 proteins that were labeled with both ICP0- and GFP-specific antibodies (yellow bands in Fig. 1D).
Many cells were positively labeled by a human-specific antibody to the progenitor marker nestin.
