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So the protesters have defied efforts to be led or labeled by either side.
p53 labeled by either anti-p-p53 or anti-p53 antibodies was immunoprecipitated by Tau1 antibodies (Fig. 3B).
A small third population of projection neurons in the ventral anterior nuclei (∼2%) was not labeled by either CB or PV.
In agreement with the analysis of the thalamic projection neurons, the vast majority of thalamo-cortical boutons terminating in layer I of prefrontal cortices were CB+ (∼82%), fewer were PV+ (16.5%), and only a few (1.5%) were not labeled by either antibody.
Cells were re-stimulated to enter the cell cycle (into G1) by addition of isoleucine-containing MEM or medium containing EGF. Replicating DNA was labeled by either pulsing for 30 minutes with bromodeoxyuridine (BrdU; 15 µM) at the indicated time points, or by continuous labeling with BrdU (20 µM) throughout the experiment.
In contrast, the majority of thalamo-cortical boutons terminating in the middle and deep layers (IIIb-VI) were PV+ (∼58%), originating from the PV+ population of projection neurons in the ventral anterior nucleus, 21% were CB+, and 21% were not labeled by either antibody (Fig. 9D, E).
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Using the MM method, sections were labelled by either anti-Pax7 or anti-NCAM for SC identification together with staining for Dlk1, laminin and DAPI.
Only 2 miRNAs were scored as present by microarray analysis using targets labelled by either the amplification or direct labelling techniques but not by the qRT-PCR assay.
The remaining 5S rDNAs were directly labeled by PCR with either biotin-16-dUTP (20 μM) or digoxigenin-11-dUTP (5 μM).
The latter have attributed the considerable variation in the percentage of labeled cells by either of [14C] and [3H] tritiated thymidine that indicates cell proliferating for similar histological cancerous tissues to errors in the technique.
In sequences of consecutive horizontal sections (0.5 µm), individual cones were either labeled by JH455 (specific to SWS1 or S-cones, gray arrowheads) or by JH492 (specific to M/LWS or M-cones, white arrowheads).
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