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As shown in Fig. 5A, PKD was labeled by both BirA*-PI4KIIα and BirA*-PI4KIIIβ; this finding is consistent with previous data showing that PI4KIIIβ is a substrate of PKD (Hausser et al. 2005).
For comparison, neurons that were labeled by both TH and DAT antibodies, and non-immunoreactive tissue within the VTA, were also collected and analyzed by PCR.
On average, 80 100% of intracellular bacteria were labeled by both LAMP-1 and LAMP-2 (Fig. 1B) suggesting that both proteins are prominently associated with the SCV and Sifs.
Single-Cys variants S47C and S313C were >70% labeled by both AF488 and TR.
In specimens displaying SNAI1 nuclear positivity the same cell population was labeled by both antibodies.
A self hybridisation (reference labeled by both Cy5 and Cy3) was carried out in another array.
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However due to the overlap between the transcripts, we were not able to determine if sites labelled by both probes expressed both AmphiGli-L and AmphiGli-S, or just expressed AmphiGli-L.
In SoFa1, all RGCs are labelled with gapRFP and therefore show a red membrane labelling; all inhibitory neurons are labelled by both gapRFP and cytGFP, and thus show a red membrane and a green cytoplasmic labelling; all BCs are labelled with gapCFP, so all show blue membrane labelling.
Tip cell MVs labelled by both CD31 and CD105 have an estimated mean diameter of 717.02 ± 130.01 nm (743.64 ± 138.93 at 9 10 wg and 702.12 ± 125.16 at 22 wg) measured on single optical planes from projection stacks at suitable intervals, as shown in the animation (Online Resource 2).
For immunofluorescence microscopy detection of cellular enzymes labeled by NC9, both Triton X-100-permeabilized and nonpermeabilized cells were used to visualize intracellular and cell surface/extracellular dansyl-labeling, respectively.
By contrast, RTCB produced heterogeneous products, resulting from re-ligation of either single exon to the intron (Fig 4D, lane 7, labeled by open arrowheads), both exons to the intron restoring the substrate (Fig 4D, lane 7, labeled by an asterisk), or exon-to-exon resulting in some fully spliced product (Fig 4D, lane 7, labeled by a closed arrowhead).
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