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Cells were labeled between 2 6 hours following infection via incubation with culture medium that was supplemented with 10 µM BrdU (Becton-Dickinson, BD).
Consistent with the idea that last order neurons were labeled between 36 40 h, we observed labeling in three populations of interneurons that are known to make monosynaptic connections with motoneurons.
To compare intracellular protein synthesis directed by the mutant FMDVs, proteins were metabolically labeled between 3 and 4 hours post electroporation. BHK 21 cells infected with mutants V3, V19 4 and V15 9 encoding R55W in 2C presented modestly higher levels of viral proteins than the same mutants expressing a wild type 2C (Figure 7).
However, the dorsalized activity of the 1xDR4 in the absence of T3, the difference in activity and cell types labeled between 1xDR4 and 2xDR4, as well as the double-shRNA DR4 activity, are all difficult to explain without invoking multiple and complex models.
Sequential actions are listed in each box, while the number of resulting reads/EDGE-tags are labeled between boxes.
Each image was randomly labeled between "A E," corresponding to a range of skin ages by decade from 20 69 years.
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One must not overlook an important difference in experience with pointing and labeling between the word-trained dogs from the current study and children.
(G and H ) Coronal sections of P18 DG and (I and J ) P30 DG showed no differences in labeling between Sema5A +/− ; Sema5B +/− and Sema5A −/− ; Sema5B −/− mice.
Variability among microarray assays might arise from differences between labeled samples, between the arrays themselves, or between hybridization conditions.
We have a set of states and a set of labeled transitions between these states.
No significant differences in numbers of labeled cells between 1% and 21% O2 were observed (p>0.05).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com