Sentence examples for labeled bands from inspiring English sources

In order to ensure that the biotin label on the initial DSS1 probe did not itself cause the formation of the protein bands, we repeated the above experiments using an in vitro [35S]-radiolabeled DSS1-myc fusion protein, employing autoradiography to detect the labeled bands.

Only unambiguously and intensely labeled bands were scored.

The labeled bands were visualized using chemiluminescence (Pierce, Rockford, IL).

The 1D4 antibody predominantly labeled bands around 40k (arrowhead in Fig. 1A), which are most likely PrUV, PrB and PrV opsins.

The identity of 59Fe labeled bands as ferritin and Tf was established by immunoblotting under native conditions as described previously [13].

Ferritin and Tf/TfR complex show corresponding iron labeled bands as expected (Fig. 3A, compare lanes 1, 2 with 5, 6, 9, 10).

However, a corresponding 59Fe labeled band is not detected in lane 2, though pure 59Fe-labeled recombinant PrP is readily detected by this method as demonstrated previously [17].

A slower migrating 59Fe labeled band corresponding to Tf/TfR complex is detected in M17 lysates (Fig. 3A, lanes 1, 7, and 9, open arrow).

When we incubated GSK3 with GST (not an expected substrate) we saw a labeled band consistent with autophosphorylation.

Solubilization with CHAPS resulted in a complete shift to a labeled band, thus excluding the occlusion of these cysteines in a protein-protein interface and demonstrating there was sufficient IASD for labeling.

In order to reduce the contrast between the labeled band and the background, less 4-OT R61C-1 waddedded to the same cell lysate, and the oxidative Heck reaction was performed.