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When fluorescently labeled and injected intravascularly into chick chorioallantoic membranes (CAMs), in contrast to unmodified viruses, the integrin-binding viral particles bound to the lumenal surface of the developing chick neovessels and infected the CAM vascular endothelial cells.
Human peripheral blood mononuclear cells (PBMCs) were CFSE labeled and injected intravenously into these mice and allowed to expand for 7 days.
Cancer cells are fluorescently labeled and injected into the embryos, and cell proliferation is monitored by live-cell microscopy.
However, in this experiment, we obtain neutrophils from a separate group of septic donor animals, labeled and injected them to all animals immediately after treatment.
For adoptive cotransfer of CTLs, day 7 fully differentiated CD4creAMPKα1fl/+ (control) and CD4creAMPKα1fl/fl (AMPKα1null) CTLs with different CD45 congenic markers were mixed at equal ratio, CFSE labeled and injected intravenously into C57BL/6 Ly5.1 or Rag2-/ recipient mice.
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Generated CTLs were magnetically labeled and intravenously injected into glioma bearing animals that underwent MRI on days 3 and 7 post- injection.
Neutrophils from septic donors (one donor for two different treated animals each time) were isolated, labeled, and then injected into animals treated with apheresis or sham (groups 3 and 4; n = 12 each) immediately after completing a course of therapy.
99mTc-ECD (ethylcysteinate dimer) was prepared by kit labeling and injected by epilepsy-trained nurses immediately after clinical seizure onset [18, 19].
To achieve such synaptology, we used only sparse retrograde labeling and injected anterograde tracers into small focal areas of cortex.
All morpholinos were FITC labelled and were injected at a dose of 20 ng in one cell of a two-cell stage embryo, followed by analysis at the tailbud stage (stage 25 28).
Autologous ADSCs were labeled and intra-articularly injected, leading to the cells populating the area of damaged cartilage as well as a decreased progression of OA [45].
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