The siGLO™ RISC-Free siRNA (non-targeting siRNA with a fluorescent label) was purchased from Dharmacon via Perbio, Cramlington, UK.
Antibodies anti-phosphoserine PSR-45, anti-phosphotyrosine PY-20 and anti-goat IgG horseradish-peroxidase-labeled were purchased from Sigma, anti-phosphotyrosine PY99 and anti-actin from Santa Cruz Biotechnology Santaa Cruz, CA, USA) and anti-mouse IgG horseradish-peroxidas e-labeled from GE Healthcare Bio-Sciences.
Isotopic labels were purchased from Cambridge Isotope Laboratories.
All isotopic reagents used for the synthesis of labels were purchased from Isotec (Miamisburg, OH).
Secondary antibodies used for fluorescence labeling were purchased from Dianova (Hamburg, Germany).
All antibodies used for cell labeling were purchased from eBioscience (San Diego, CA, USA).
Polyclonal anti-collagen type II antibody (PAB746), monoclonal anti-adult cartilage-specific proteoglycan antibody (MAB2015), anti-β1-integrin antibody (MAB1965), and alkaline phosphatase linked sheep anti-mouse (AP303A) and sheep anti-rabbit secondary antibodies (AP304A) for immunoblotting and immuno-electron labelling were purchased from Chemicon International, Inc. (Temecula, CA, USA).
Indium-111 used for labeling was purchased in the form of 111InCl3 in a solution of 0.05 M HCl (0.5 mL) from Mallinckrodt Medical B.V. (Petten, the Netherlands).
Fluorescently labeled streptavidins were purchased from BD Bioscience.
Caspase activity substrates, AnnexinV- Alexa 488 conjugate and Vibrant Lipid Raft labeling kit were purchased from Molecular Probes/InVitrogen.
