(A ) Label transfer assays with P-labeled tRNA-ProUGG were performed in the presence of various concentrations of RCMLA.
(B ) Label transfer assays with P-labeled mature tRNA-ProUGG were performed in the presence of various concentrations of ATP.
(E ) Label transfer from P-UTP-labeled tRNA-ProUGG to Ssa2p was monitored in the presence of increasing amounts of competitor tRNAs transcribed in vitro or purified from yeast.
In contrast to saturation, frequency-labeled exchange transfer (FLEX) [31 33] uses a series of Label Transfer Modules to selectively label the exchangeable protons that could be subsequently transferred to water.
Both Ssa1p and Ssa2p received radioactivity from P-labeled (AUUU 5 RNA but not from (ACCC 5 RNA, and label transfer was dependent on UV-irradiation and sensitive to ATP.
In particular, tRNA-ProUGG was a potent substrate for label transfer while tRNA-PheGAA was a very poor substrate irrespective of P-labeled nucleotides used for in vitro transcription.
(2) Conduct label transfer in an efficient nearest neighbor search and a temporal MRF model.
We present CollageParsing, a nonparametric scene parsing algorithm that performs label transfer by matching content-adaptive windows.
Performing label transfer using content-adaptive windows enables the construction of a more effective Markov random field unary potential than previous approaches.
The proposed method combines the depth clue with traditional RGB information and is divided into three steps: depth recovery and superpixel generation, weakly supervised SVM classification and context-aware label transfer.
