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Fluorescent dye DiR was applied to label the cell membrane of human CIK cells labeled with Au nanorods.
4′,6-Diamidino-2-phenylindole (DAPI) was used to label the cell nucleus before imaging with a fluorescence microscope.
From Figure 7, we can see that the QDs can enter the cytoplasm of living ECV-304 cells to label the cell [56].
Moreover, to label the cell mitochondria for locating Cur/TPP-oHA-S-S-Cur micelles and Cur/oHA-Cur micelles within cells, the cells were further incubated with a mitochondrial tracker (Mito-tracker Red CMXROS) for 15 min.
CTCR8 was found to label the cell surface of AChR100-116-specific T cell lines and clones, immunoprecipitate the TCR from such cells, and block their proliferative responses to AChRα100-116.
Red color is from rhodamine B, which was used to label KLH; green color is from NBD PE, which is a fluorescent lipid used to label the lipid layer; and blue color is from CellMask™ Blue Stain, which was used to label the cell membrane.
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"With our new approach, we look to label the cells with an iron supplement and we can see the transplanted cells directly," said Dr. Heike Dandrup-Link, authorhof of the study, to the San Francisco Business Times.
Cells were then washed and incubated on ice with FITC-conjugated goat anti-mouse immunoglobulin to label the cell-bound mAbs or liposomes.
Cells expressing MarsCy1-Lgr5-EGFP were co-pulsed with HA-antibody and SCi1 at 4 °C to label the cell-surface pool of the receptor (Fig. 1b– d and Additional file 2: Figure S2).
To assess more directly whether GLUT4 translocation was affected by the RNA silencing of Rab3, we used the glucose transporter affinity tag Bio-ATB-BGPA to label the cell-surface exposed GLUT4.
Chauvigné-Hines and co-workers used a suite of GH-ABPs (difluoromethylphenyl aglycone, 2-deoxy-2-fluoroglycoside, and N-halogenated glycosylacetamide) to directly label the cell-adherent cellulosome of the model anaerobic thermophilic organism Clostridium thermocellum [ 28].
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