Cells were suspended in FACS buffer (PBS, 0.2% bovine serum albumin, 0.1% sodium azide) and labelled with the appropriate concentration of anti-m5T4 monoclonal antibodies diluted in FACS buffer for 30 mins on ice, washed with FACS buffer and labelled with rabbit anti-mouse IgG conjugated to FITC (Dako, 1∶40).
A PCB could be labeled with the appropriate leads, or a battery system with polarities.
The number in the upper string indicates the atom number, which was labelled in the appropriate SoLA.
The suspension of released cells was collected by centrifugation, washed and surface labelled with the appropriate fluorochrome-conjugated monoclonal antibodies (mAbs): CD45-PerCP, CD3-FITC, TcRγ δ-PE and CD103-PE (BD Biosciences, San Diego, CA).
As control, we used isotype-matched antibodies labeled with the appropriate fluorochrome (BD Biosciences).
For generation of the labeled DNA, the appropriate operator fragment of target genes (dr1709, dr1998, dr2263, drB0036, and drB0125) was amplified by PCR with genomic DNA and cloned into pMD18, followed by FITC-labeled RV-M and reverse priming of target genes.
Slides were labelled with the appropriate specimen codes and placed on the magnetic workstation.
Next, cells were labeled with the appropriate fluorescent-tagged secondary antibodies.
Blood anticoagulated with PPACK was labelled with the appropriate antibodies exactly 5 min from collection and incubated in the dark for 20 min at room temperature.
