Sentence examples for label the active from inspiring English sources

These ABPP probes selectively label the active forms of enzymes followed by quantitative analysis to characterize changes in enzyme activity without corresponding changes in protein expression.

Activity-based probes (ABPs) label the active form of an enzyme via covalent modification of catalytic residues.

The aim of this study was to develop an agonist positron emission tomography (PET) ligand in order to label the active G-protein coupled 5-HT4-R in peripheral and central tissues.

Fluorescent inhibitors have also been developed to specifically label the active sites of the assembled proteasome.

This can be taken as an indication that the agonist PET ligand preferentially labels the active G protein-coupled receptor pool, which is the target for the endogenous neurotransmitter.

On Western blot, ab3 detected both pro-MMP-3 (55 kDandactivetive MMP-3 (47 kDa), while ab4 only labeled the active form of MMP-3 in retinal homogenates (Fig.  2d).

Labelling the active site cysteine with a bromacetanilide conferring selectivity included a C label and also F. The bromacetanilide binds covalently to the active site Cys (determined by mass spectrometry) and we hedged our bets with the possibility of probing the molecular environment of the active site by C and F NMR should it prove impossible to obtain crystals.

Western blotting of retinal tissue homogenates pointed out that both antibodies labeled the active, soluble form of MT1-MMP (50 53 kDa) that is shed from the cell membrane upon cleavage in the stem region of mature MT1-MMP, while the active, membrane-bound MT1-MMP (mature MT1-MMP, 57 kDa) was only recognized by ab8.

They usually consist of two key elements (a reactive group for binding and covalently labeling the active sites of a given enzyme class or classes; and a reporter tag for the detection, enrichment and identification of probe-labeled enzymes in proteomes), such as fluorescence or MS [ 32, 33].

Activity-based probes (ABPs), which target and covalently label the enzyme active site with a fluorophore, have also been described and used to assess the functional proteomics of the proteasome.

In contrast to common probes that possess electrophilic reactive groups that can label the conserved active site nucleophiles of specific classes of these enzymes, such as the serine and cysteine proteases and subunits of the proteasome, MPs use a zinc-activated water molecule for catalysis.