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Oligonucleotide probes were 32P-labeled using T4 Polynucleotide Kinase 5' End Label System (Promega) and purified using Mini Quick Spin Oligo Columns (Roche).
Myspace was never a substitute for the record label system.
Here, then, is a potted history of one of the bumpiest rides ever through the record label system.
The UK's AIM awards honour the artists, festivals, record companies and executives who flourish outside the major label system.
It was streaming that was to give him the impetus to leave the major label system and set up 300 Entertainment in 2013.
Street rappers outside of the major label system have for years sidestepped stressing about "the album" and instead focused on mixtapes, club shows and other sources of income.
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Despite the general tenor of his remarks, Green underlined that he still supports the label system.
Jay-Z, for his part, suggested that the string of stars to exit the major-label system would also signal to younger acts how to plot their careers.
"I'm leaving the major-label system, and there are hundreds of artists watching my court case and wanting to follow me," Ms. Love declared.
It's the album she has been announcing she would release for some time, made outside the major-label system and its pressures to design songs for pop radio.
In 2004, when she released the mixtape "Piracy Funds Terrorism" — a clattering, irresistible whirlwind of squelching baile funk, reggaeton, and hip-hop — the major-label system was largely intact.
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