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Figure 4 Label stability of the FMNP-labeled MSCs.
These residues, however, are prone to deiodination after in vivo administration [28] and generally result in low label stability and loss of free iodide.
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He offered only the barest proposals for political change, instead labeling stability as "a principle of overriding importance" and calling for more shared decision-making within the "people's democratic dictatorship".
Labelling stability of WBCs 240min after cell labelling was above 91% but decreased to 78.1% 48h after labelling.
The optimized labeling procedure is described in Fig. 3 aDecay corrected Fig. 2 Post-labeling stability analysis.
Labelling stability of WBCs 240min after cell radiolabelling was above 68% and remained constant up to 48h after labelling.
The labelling stability and biodistribution of the tracer was first evaluated in control mice (n = 10) 1 h post injection (p.i).
Labelling efficiency, cell viability, assessed by Trypan Blue exclusion assay and labelling stability (efflux of radioactivity) was determined at different time points after radiolabelling.
The in vitro labelling stability was evaluated using SDS-PAGE analysis (100 V constant) on NuPAGE 4-12% bis-Tris gels (Invitrogen) with the aims to check the release of 111In and transchelation of 111In to serum proteins.
Post-labeling stability was assessed by calculating the release fraction of radioactivity and identifying the chemical forms of 18F in the released and intracellular components of 18F-FDG-labeled RBCs incubated in plasma.
Regardless of its sufficient labelling stability with DTPA, 111In is associated with photon energies that are suboptimal for in vivo imaging and long half-life resulting in relatively high radiation burden, which both represent significant limitations for the translation of this radiolabelling approach to clinical studies.
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