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The label of stem cells requires labelling methods making grafted cells distinguishable from host cells to follow transplanted stem cells by the above methods.
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An alternative antibody-based stem cell labeling method employed 89Zr-labeled anti-CD45 for ex vivo labeling of stem cells expressing CD45 membrane protein.
Labeling of stem cells aims to distinguish transplanted cells from host cells, understand in vivo fate of transplanted cells, particularly important in stem cell therapy.
These reports have generally used direct ex vivo labeling of stem cells with FDG [15, 16] or indirect labeling using a reporter gene such as herpes simplex virus type I thymidine kinase (HSV1-tk) [17, 18].
This approach to design and tailor new method for labeling of stem cells may be useful to provide better understanding on the therapeutic effects of transplanted stem cells into the target diseases.
For labeling of stem cells, 50 mg/kg bromodeoxyuridine (BrdU) neutralized in PBS with 0.007N NaOH was administered intraperitoneally at the time of surgery and at 12 hour intervals for 7 days.
However, these conventional labeling methods have limitations for use in labeling of stem cells [ 9, 11].
The molecular basis for selective MMTV-driven labeling of stem cells and pregnancy-activated progenitors remains to be elucidated.
This study suggests that bioconjugated quantum dots are a viable probe for long-term labeling of stem cells [ 89].
In addition, long-term labeling of stem cells is critical to elucidate their fate, migration, and contribution to regenerating tissues.
Such long-term labeling of stem cells will be beneficial for elucidating the relative contributions of native and engineered tissues to morphogenesis.
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