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This is because students who study internal or general medicine, both of which fall under the label of primary care, often go on to specialties after they complete their residency.
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The novel method employs the two-step process (Figure 1): 1) preparation of 89Zr-labeled p-isothiocyanato-benzyl-desferrioxamine (89Zr-DBN) and 2) random labeling of primary amines of cell surface proteins with 89Zr-DBN.
These results suggest that 99mTc-HMPAO labelling of primary human monocytes does not strongly interfere with mononuclear cell viability and function.
Labelling of primary DG cells with the mitotic marker BrdU suggested that postmitotic young neurons are the source of the transplanted mature neurons in-vivo.
Purified proteins were dialysed into PBS prior to fluorescent labeling of primary amines with Oregon Green succinimidyl ester according to manufacturer's instruction.
In the experiments that involved the labelling of primary cells with the mitotic marker bromodeoxyuridine (BrdU), a single injection of BrdU (200 mg/kg in saline) was given IP 5 7 hours before the animals were euthanized.
Based upon our previous experience with a spotted filter-array-based nuclear run-on (NRO) using radiolabeled UTP[15], [16], our initial goal (Figure 1a) was to substitute a non-radioactive labeled nucleotide (biotin-UTP) during the metabolic labeling of primary nuclear run-on RNA in order to select for newly transcribed (nascent) RNA only.
Western blotting with surface-biotin labeling of primary T cells and histological studies with confocal microscopy of MS4a4B-expressing retrovirus-infected EL4 thymoma cells showed that MS4a4B was indeed expressed on cell surface (Fig. 1A and B), suggesting that MS4a4B may potentially interact with other cell membrane proteins in T cell regulation.
Relative quantification of digested peptides was performed by stable isotope labelling of primary amines as previously described [ 19].
Specifically, anti-AT labeling of primary motor axons did not show consistent defects over the length of the trunk.
Global labelling of primary endocytic vesicles was achieved by incubation of cells with sulfo-NHS-SS-biotin at 4°C, warming to 37°C by rapid buffer exchange, and allowing uptake for 20 s.
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