The use of lipophilic carbocyanine dyes, combined with particle-mediated biolistic delivery allows for non-toxic fluorescent labeling of multiple neurons in both living and fixed tissue.
Rapid technological progress in serial immuno-staining14, genetic fluorophore engineering15, and spectral unmixing16 now allow simultaneous labeling of multiple subcellular components, requiring a co-evolution of the digital representation system.
We have developed two protein-mediated protection schemes that permit independent labeling of multiple thiols.
Finally, the method is flexible and easy to use for the fluorescent labeling of multiple proteins or for pulse-chase labeling.
The mutual orthogonality of the SNAP- and CLIP-tags enables the simultaneous labeling of multiple proteins in the same cellular context.
Looking in detail at the Alizarin Red labeling of multiple preparations at successive stages shows how different modes of outgrowth contribute to the bone's changing form.
Pioneering work in the chick hindbrain, involving labelling of multiple neuroepithelial cells with a lipophilic dye, identified cell lineage restriction boundaries at the borders between rhombomeres [8].
These samples are more complex than parasite lysates and contain a number of highly related papain fold cysteine proteases, in addition to the DPAP1 homolog Cat C. As expected, addition of FY01 to rat liver lysates resulted in labeling of multiple proteases targets (Figure 3A).
In addition, detection requires labeling of multiple probes or incorporation of fluorescent dye- or biotin-conjugated nucleotides into double-stranded DNA (dsDNA) generated by RT-PCR.
Immunofluorescence allowed for simultaneous labeling of multiple signals within the human skin sections that included nerves, mitochondria, and nuclei (Fig. 2A).
This allows linker 5 to be used as a synthetic intermediate for one-step NIR/Cu labeling of a variety of peptides, and could allow the simultaneous labeling of multiple peptides with just one batch of Cu- 5.
