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The label is administered at one selected time point, and development is allowed to continue.
Variables to consider when designing cell proliferation studies include the animal's age, chemical dose and method of treatment, choice and dose of label, time and length that the label is administered, and methods of quantitation.
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No label was administered for the next 3 weeks.
Nuclear label retention for extended periods in individual cells may be explained by the presence of originally labeled cells that went out of cycle shortly after the label was administered.
As mentioned above, [H]-thymidine retention defines the long label retaining cells in group 4, and 5BrdU incorporation defines the cells traversing the cell cycle when this label was administered on days 11 and 12 of pregnancy.
Nuclear label retention for extended periods in individual cells may be due to the presence of labeled cells that differentiated and exited the cell-cycle shortly after the label was administered or may represent quiescent cells that divide infrequently or traverse the cell cycle very slowly.
To asses the dynamics of bone formation, fluorochrome labels were administered and histomorphometry focused on the distribution of bone in the scaffolds.
The labels were administered through tail vein injection.
The labels were administered within one hour after wound creation for all images in this figure.
Fluorescent labels were administered to the beagles to monitor new bone formation around scaffold.
When both labels were administered in vivo, there was good agreement for Ts between the FCM and IHC methods.
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