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Differentiation happens when a cell changes its phenotype to that of its direct progeny - independent of cell division and hence label intensity is unaffected.
We thus know that label intensity
Following 6 days of chase, the reduction in biotin label intensity is similar in WT and 5A3 +/del CD150hi HSC, CD150lo HSC, and CD150neg MPP.
This means that cells of highly different division number can have similar label intensity, whereas two cells of the same division number can possess highly differing label intensities.
Figures show histograms of per molecule map quality metrics including length, molecule map SNR and intensity, label count, label SNR and label intensity.
By comparing these hybridization sites in terms of their linkage groups and label intensity, the authors inferred the likely orthologies of tRNA loci.
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The output of labeling experiments consists of a sample of measured label intensities { l(v )}, v = 1,..., M, with M the number of measured cells.
A cell that self-renews has undergone cell cycling and hence is replaced by two daughter cells with changed label intensities.
Label intensities are observed at the location of each probe.
In this phase, separated peaks occur, but only at very low label intensities.
This shows that there is no trivial relation that would allow to deduce proliferation properties directly from label intensities.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com