The ultrasound and microbubble treatments given, followed by 8 Gy, indicated a significant increase in γH2AX labeling, indicative that tumor cells might be potentially sensitized by the vascular insult caused by ultrasound-stimulated microbubble enhancement of radiation effect.
For detection of necrotic cell death, the cells were placed on ice and incubated with 50 μg/ml propidium iodide (PI; Sigma-Aldrich) in PBS for 10 min. The samples were analysed directly by flow cytometry for PI labelling indicative of cellular permeabilization.
Mammary cells within the ducts and alveoli that retain both a 5-BrdU and H-TdR double-label are indicative of long-lived label-retaining cycling epithelial cells (LRECs).
For the 58 out of 108 apparently unidirectional pathways in which more than 10 neurons were labeled, 32 showed an overlap between the injection site and labeled neurons indicative of a genuine unidirectional projection (see Supplementary Table 4).
Samples with high census (index 4-5) always contained some L2d and dauer larvae (labeled P2), and some did not contain L3 and L4s at all (labeled P3), indicative of populations entering the dauer stage at the end of a proliferative stage.
The subscale labels are indicative of their underlying constructs.
The BrdU labeling index, indicative of proliferation in cells, is directly related to the number of cells in S phase.
Fractions F8 and F9 contained relatively few S-phase cells (8%–14%) with labeling patterns indicative of early S phase (I and II).
Our results suggest that high RIZ1 labeling is indicative of lower grades of gliomas and is associated with better progression-free and overall survival rates.
Lesions that were positively correlated to at least two definite variables were labeled as indicative traits of scurvy, and when at least two of these indicative variables were present they formed the basis of a probable diagnosis of scurvy.
Given that axonal tracers-like BDA undergo fast axonal transport, this questions the interpretation of tract tracing data; is increased axonal labelling really indicative of a greater number of axons or does it merely reflect increased axonal transport?
