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The discs contain some of the best jazz and R&B of the time, including Johnny Otis and his Orchestra, Slim Gaillard, Charlie Parker, Erroll Garner, Helen Humes and Big Jay McNeely; the purview of the label incorporated bebop's subtleties but tended more toward frantic good times.
A 13C-labeled octanoic acid breath test method was used to measure gastric emptying, with the label incorporated into the non-nutritive paste.
Although the total number of proteins identified with high confidence (578) was relatively small compared to other proteomic studies in yeast, (most likely due to the charge-neutralizing effect on peptide n-termini of the PIC label incorporated for quantitative analysis [23]), we were able to identify abundance changes for 127 proteins upon rapamycin treatment.
To label incorporated EdU, cells were incubated for 30 min with TBS, pH 7.2 containing 4 mM CuSO4, 1.94 mg/ml sodium ascorbate, and 4.5 ug/mL Alexa-fluor 488-azide (Invitrogen A10266), washed with PBS, and incubated overnight at 4°C with primary antibodies in 1% BSA/PBS (Cyclin A, ab16726, Abcam, Cambridge, MA; phospho-histone H3, #3377, Cell Signaling Technology, Danvers, MA).
We found no label incorporated into dehydolathosterol or desmosterol.
The detection method was PCR in situ hybridization, in which the PCR occurs in situ but with no label incorporated during amplification (15 ).
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In addition, we were able to construct peptides that had photo-affinity labels incorporated into them, which allowed the peptides to be covalently attached to the MHC molecules after binding.
The rows labelled 'Incorporated into the intervention' indicate the final decision about the nature of the intervention along the 'core components'.
This indicates that the pulse of EdU labeling incorporated into the (replicating) cells adjacent to the disc epithelium was now manifest in the (non-replicating) cells located in more distal layers.
In the case of named entity recognition, the labels incorporate two concepts: the type of the entity and the position of the token within the entity.
During pulse-chase experiments, as the replicating forks move away from the labelled regions, the label is incorporated into high molecular weight, continuous DNA and is observed as a comet head.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com