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We have used a modified method, injecting a higher dose than recommended on the label in multiple spots in the cord in one session (Atroshi et al. 2014).
(B and D ) Histological sections stained with antibodies to GFP (brown) confirming absence of label in chicken fibroblast injected animals (B ), and presence of label in multiple tissue types in the iPSC-like injected animals (D ).
These cases were selected as each had frontal label in multiple sites, and they respectively involved efferents from the anterior (ACy12 and ACy14), mid (MLP-L and MRC), and posterior (ACy28) levels of the hippocampus.
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To calculate the probability of mitochondrial localization, we counted the mitochondrial gold labeling and compared them to the cytoplasmic labeling in multiple images of the experiment versus the control (chi-square test).
We assume that the neurons not responsible for the phenotype will be labeled in some but not other GAL4 lines producing the same phenotype, whereas the essential neurons will be more consistently labeled in multiple GAL4 lines producing the same phenotype.
Yearly sales of rituximab in the US already exceed $2 billion, attesting to its increasing US Food and Drug Administration (FDA -approved and oFDA -approvedin multiple clinicandsettings such as B-cell lymphoma, lymphoproliferative disorders, and refractory inflammatory conditions, incluseng rheumatoin arthritis (RA) (Table 1) [ 1- 5].
"Off-label drug use in multiple sclerosis" or "off-label use of beta interferon" might serve as an example.
In ontologies, labels may be provided in multiple languages, and multiple labels may be assigned to one class.
Regarding Pouch, Global Savings Group says it will incorporate the product into its white label offering for publishers in multiple markets.
To this end, we compared the number of gold particles, reflecting mitochondrial labeling by the anti-TF antibodies, with the number of gold particles reflecting cytoplasmic labeling by these antibodies, in multiple images and experiments (for details, see Materials and Methods).
The company started by constructing essentially a crowdsourced mechanical turk for labeling words and sentences in multiple languages.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com