Subsequently, we performed in vitro experiments using a 3-cell type system (ECs, MPCs, and pericytes (PCs)) to demonstrate the utility of fluorescent cell labeling for monitoring cell growth and differentiation.
It is expected to have many potential applications in protein engineering and research, which include fluorescent labeling for monitoring protein folding, location, and trafficking in cells, and biotinylation for protein immobilization on streptavidin-coated surfaces including protein microchips.
Our initial findings of distribution of 89Zr-labeled hMSCs in mouse models confirm the biostability of the radiolabel bound to the DFO moiety supporting further exploration of the 89Zr-DBN labeling method for monitoring stem cell engraftment and cell trafficking.
Using the HaloTag system, Stagge et al. developed an electroporation-based labeling technique for monitoring individual proteins in yeast with super-resolution microscopy.
Nevertheless, fluorescence imaging constitutes an attractive imaging modality for research purposes, as it uses stable labels suitable for monitoring animals over extended periods of time and allows direct validation through fluorescence histology, and translation to clinics can be readily achieved by replacing the fluorescent label with a radionuclide label.
The specifics of quantum dots use as fluorescent labels for continuous monitoring under constant UV illumination were outlined.
Once enrolled, calves were assigned a case number and the hutch was labeled for subsequent monitoring.
Fluorescent probes are extensively used and can be found expressed in reporter proteins (GFP, RFP), in microspheres or as dyes that are widely used for labelling cells or for monitoring gene expressions.
We established a label-free platform for monitoring the changing morphology of live cells in real time based on scanning electrochemical microscopy (SECM).
Syto9/PI labeled biofilms allowed for monitoring the viability of bacterial populations as a function of the membrane integrity of the cell.
