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In the best case, this would include an EM label for human vs. mouse cells – immuno-EM, presumably.
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We determined PCNA-labeling (for mouse tissues), Ki-67-labeling (for human tissues) and ubiquitin-labeling indices by counting immunostaining-positive cells after counterstaining with hematoxylin.
Human cells were identified by labeling for human cytoplasmic marker STEM121.
Tapentadol (TAP) is a novel atypical opioid drug labelled for human use.
Nootropics in this class are sold on the open market as "research chemicals" and cannot be labeled for human consumption.
Serial sections were labeled for human nuclei to ensure no human neurons were included in the NeuN cell counts.
We investigated both immature and mature oligodendrocyte differentiation by double labeling for human nuclei and Olig2 to assess differentiation to immature oligodendrocytes (Fig. 4A E) and APC-CC1 to examine mature oligodendrocytes (Fig. 4F J).
Mice were necropsied at 1, 3, 6, 9, and 12 months and TA sections were labeled for human dysferlin expression.
Finally, the multiplexed phosphorylation TKR assay is labeled for human phosphorylation receptor detection as only approximately 50% of the phosphotyrosine receptor antibodies have been validated for canine use.
Aliquots of 200 ng total RNA were amplified and labelled for Human Gene 1.1 ST 96-Array (Affymetrix) using the Ambion WT Expression Kit and GeneChip WT Terminal Labeling and Controls Kit (Affymetrix), according to the manufacturer's protocols.
To verify if the dendrites imaged in vivo belonged to neurons expressing the human tau transgene, we performed immunohistochemical labelling for human tau on brain slices (antibody HT7; Figure 3a).
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