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In this work, a highly sensitive sandwich-type electrochemical immunosensor was fabricated by using the gold nanorods (Au NRs) as the substrate material and the mesoporous Au@Pd@Pt core-shell nanospheres (m-Au@Pd@Pt) as the label of the detection antibodies (Ab2, Ab2 label) for detecting Hepatitis B surface antigen (HBs Ag).
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The application of nanotechnology and nanomaterials enhances the availability of numerous detectable labels for detecting a wide variety of analytes with a low limit of detection, and leads to the development of ultrasensitive and ultraminiature devices for immunoassay.
Chen et al. applied FDA-drug labels for detecting drugs that can induce liver injury [ 7].
The genotyping assay contains two unlabeled primers (forward and reverse) for amplifying the sequence of interest and two labeled probes for detecting alleles.
The TUNEL (terminal nucleotidyl-mediated nick end labeling) assay for detecting the status of DNA damage in seminiferous tubules of rat testis, at different stages of chromatin condensation, has been done according to the method of Leduc et al. [ 24].
On the whole, cell¿s mechanical properties represent the physical structure of living cells and have been proved to be effective as label-free biomarkers for detecting abnormalities in diseased cells [7].
TdT-mediated dUTP nick end labelling was used for detecting apoptosis.
Using the terminal deoxynucleotidyl transferase dUTP nick end-labelling (TUNEL) assay for detecting apoptotic DNA fragmentation, we demonstrate that a large proportion of A. queenslandica larval epithelial cells undergo programmed cell death shortly after the onset of metamorphosis [see Additional file 2: Figure S2].
However, single amplification by enzyme labels is not sufficient for detecting an ultra-low analyte concentration.
In this study, we describe an enzyme-functionalized label-free immunosensor system for detecting fish cortisol levels.
In this paper, we report a novel label-free electrochemical aptasensor for detecting proteins in whole blood based on a three-component nanocomposite, in which ferriferrous oxide and three-dimensional graphene nanocomposite were modified with the plasma-polymerized 4-vinyl pyridine (Fe3O4@3D-rGO@PP4VP Fe3O4@3D-rGO@PP4VP
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