mRNA was isolated and the labeling and expression profiling using Affymetrix arrays (genechip mouse genome 430 2.0 array) was performed as described previously (Boros et al. 2009).
Stem cell subclass was determined by piwi-1 labeling and expression of soxP-1 pooled with soxP-2 (sigma subclass), hnf4 (gamma), or zfp-1 (zeta).
Because of the inherent nature of the infrared fluorescence imaging system, we present only the ratio of labeling and expression in arbitrary units.
Large-scale clinical genomic studies often lead to unique analytical challenges, including dealing with a large number of clinical variables, unclear clinical endpoints or disease labels, and expression heterogeneity (Leek and Storey, 2007).
The main effects were qualified by a significant two-way interaction between colour label and emoticon expression, F 4, 937) = 2.80, p = .025, η = 0.011 (see Fig. 3).
Details of the methods for RNA quality, sample labeling, hybridization, and expression analysis were according to the manual of Affymetrix Microarray Kit.
It is important to note that, because of the low labeling densities and expression levels needed for this imaging approach, we cannot directly infer a causal relationship between FcεRI immobilization and dynamin recruitment.
Antibody staining of β-galactosidase revealed a strong expression in mTEC (Fig. 1E) as defined by MTS10+ labeling and weak expression in cTEC (Fig. 1D) as defined by β5t+ labeling consistent with our qrtPCR data.
To solve these problems, this paper proposes a novel distributed content filtering algorithm based on data labeling and policy expression (DCF-DLPE).
In contrast, N-terminal deletions exhibit no changes in amount of apoptosis compared to wild-type Id2 transfectants based on Tunnel labeling and Bax expression and an increased percentage of sub-diploid DNA content [21].
