Differential labeling analysis using getIsoDiffReport and the additional filtering subroutines described in the Experimental Section revealed that 95 groups had statistically significant different labeling patterns (p < 0.05) in control versus LPS-challenged cells.
Hydrocarbon chain mobility was measured using fatty acid spin labeling EPR analysis using 5-nitroxyl stearate (5-DSA, Aldrich) as a spin probe [76], [77].
In order to unravel the spatial distribution of Sun1 in spermatids, we performed double-label immunofluorescence analysis using affinity purified antibodies to Sun1 together with antibodies to Lap2, Lamin B3 and Cage1.
In order to further characterize these differentiated PNS neurons we performed double labeling immunofluorescence analysis using combinations of peripherin with Brn3a antibodies (Figure 2N P) or peripherin with Islet-1 antibodies (Figure 2Q S) in order to establish their sensory identity as described previously [24].
Total RNA was processed and labelled for microarray analysis using the One cycle target labelling and control reagents kit (Affymetrix, Santa Clara, CA), and used to interrogate the Affymetrix rat 230 v2 GeneChip set, representing over 30,000 transcripts including approximately 28,000 well substantiated rat genes.
Total RNA was amplified and labeled for microarray analysis using the AminoAllyl Message Amp II aRNA amplification kit (Ambion) following the manufacturer's specifications.
Taken together with the results of labeling and rescue analysis using P0-Cre, medial migration of NCCs into the spinal cord seems a plausible mechanism for the emergence of intramedullary EGFP-positive cells in Six1/4 EGFP/EGFP.
After annotating the peptides and proteins identified in each gel band, label-free quantitative analysis using spectral counting, LC/MS extracted ion currents (XIC), or MS/MS total ion current (TIC) can determine which identified protein has been enriched in your DARTS experiment (112– 112).
Similar analysis using labels at positions 345 and 905 of the amino and carboxy terminals, located them slightly above the planes delimited by the amino and carboxy end labels, respectively.
These improvements could include the use of fluorescently labelled primers and fragment analysis using a DNA sequencer to accurately assign repeat sizes.
