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As we considered only alternatives derived from RefSeq proteins, we could miss some alternatives and thus label a fraction of the alternative regions as constitutive.
We partition the transcript pool as follows: Label a fraction f g of genes in the transcript pool as active and the remainder as silent.
Label a fraction f t of transcripts belonging to active genes as 'expressed' and the remainder as 'unexpressed from active genes'.
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(D ) X-gal staining of Smarcd3 -F6-nlacZ allele (light blue) labels a fraction of total Smarcd3 mRNA.
Here, we found in the developing CrxGFP retina that Rxrγ co-labelled a fraction of GFP-expressing photoreceptors consistent with a marker of developing cone cells (Supplementary Material, Fig. S1A D").
To test for clonality of TDLU-like structures, we labeled a fraction of freshly isolated HMECs with eGFP or mCherry fluorescent protein by lentiviral transduction before plating cells in several concentrations.
For T4 DNA polymerase end-labelling a fraction of the purified PCR product was combined with reaction buffer to a final concentration of 1X, 100 μM dATP, dTTP and dGTP, 5 μCi [α-P] dCTP (3000 Ci/mmol), 1 unit of T4 DNA polymerase I and water to a final volume of 20 μl.
As shown in Figure 6C and Supplementary Material, Figure S3B, we observed that the EGFP-MC-LT fusion protein distributed in a ring-like pattern in most transfected cells and was found to label a high fraction of melanosomes in both Oa1+/− and Oa1−/− melanocytes (Fig. 6C), as well as Oa1−/−LOA1SN and Oa1−/−LOA1Δ18SN melanocytes (Supplementary Material, Fig. S3B).
This is in line with the observation that virtually all TDP-43-negative inclusions in C9orf72 patients contain poly-GA, while antibodies against the other DPR species label only a fraction (10 50 %) of these inclusions [ 35, 36].
We therefore isotopically labeled a defined fraction (10%) of either ammonium or nitrate and quantified the relative amount of 15N label in the final biomass.
In most conditions, this method labels only a fraction of input neurons and would thus benefit from improved efficiency of transsynaptic spread.
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