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Notably, the K-10 strain in our lab was obtained from Dr. Raul Barletta, the same origin for the K-10 strain used in the genome project (personal communication).
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The pBabe-hygro p53DD construct from Dr. Bob Weinberg's lab [42] and the pBabe-puro-cyclinD1-HA from Dr. William Hahn's lab were obtained from Addgene (Addgene plasmids 9058 and 9050 respectively).
LAB were obtained by performing the low-speed and high-speed centrifugal steps as described on the liquid fraction obtained after initial hand squeezing of the retrieved rumen sample.
Routine prenatal labs were obtained, and routine vaccines were administered.
The results showed that only 1 LAB isolate was obtained.
Moreover, the complete distribution of proteolytic system components in various sequenced LAB strains was obtained.
*The mean cost for lab tests was obtained by surveying the CPT fee codes contained in the CMS database.
The tubes were then transported to the field lab, and plasma was obtained by centrifugation each evening.
Larger lab-scale peptide production was obtained using the Wave Bioreactor™ 20/50 EHT system (GE Healthcare, Brazil).
The questionnaire pack along with an information sheet explaining the purpose of the study was distributed to the students during class time (either in lecture theatres, classrooms, or labs) and verbal consent was obtained.
E. coli XL1-Blue for transformation was obtained from lab stock.
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