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Then, the candidate polymorphic regions were subjected to lab validation.
The markers present in our database need further wet lab validation.
Most importantly, wet lab validation of 8 predicted extensions confirmed all of the in silico predictions.
Finding of these in silico mined STR markers needs extensive wet lab validation across all important tomato varieties of the world.
Though we have reported small attempt of in silico validation in our studies but extensive wet lab validation of these markers is warranted.
An fsCoP biomarker may be intriguing for further wet lab validation, since it satisfies both the classification optimization function and the biomedical knowledge.
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PKa has done wet lab validations.
Notwithstanding these issues, the structures that overlap between this study and others are of higher importance for further wet lab validations (Additional file 4).
This bioinformatics study lays a ground work for future wet lab validations of putative RNA stem-loop groups and represents a framework which can be used to analyze RNA structures identified by other approaches and in other species.
Intra-lab validation by two different operators confirmed the specificity and LOD of this multiplex PCR method.
His is current research efforts focus on development and inter-lab validation of an analytical method for determination of aflatoxin M1 and B1 in liver.
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