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It is therefore important as well to evaluate the performance of such LAB expression systems with genes of taxonomic distant origin.
In this regard, it is important to note that research on LAB expression systems is still in progress, and it can reasonable be expected that expression efficiencies of such systems will be much improved over the next years.
In conclusion, with dkr from C. glutamicum as example, our results confirm that LAB expression systems such as NICE and pSIP are indeed attractive candidates for high level protein production and may gain further interest for industrial purposes in the near future.
Future studies could address this problem using array and wet lab expression data.
Other LAB expression systems are inducible under various conditions such as phage attack, temperature or pH shift, or the presence of specific sugars.
The gene is expressed in copper cells, but not elsewhere in the larval midgut, and we observed similar specificity of lab expression in the adult.
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At stage 8, CapI-lab expression is downregulated in all tissues.
Esophageal CapI-lab expression is limited to the posterior portion of the esophagus.
CapI-lab expression expands to include all segments, with highest levels in T2 and T3, but is absent from the posterior growth zone.
Once the larva is competent to undergo metamorphosis at stage 9, CapI-lab expression is no longer detectable in the head (Figure 3E and 3F).
At this stage, segmental CapI-lab expression in the epidermis is in discrete ventrolateral and dorsolateral patches, with additional lateral patches in the four anterior-most thoracic segments (Figure 3C and 3D).
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