Previous computational models developed in our lab applied finite element simulations to approximate photothermal heating of gold nanorod-containing subcutaneous tumors using the bioheat transfer equation.
Briefly, the sections were rinsed with Tris buffer saline (TBS) and the biotinylated antibody from the universal streptavidin-biotin kit (Vector Lab) applied for 30 min, before rinsing with TBS.
Senior scientists working away in their labs, applying for new research grants, writing new papers, hiring new postdocs, remain as oblivious as ever, and as unlikely to note the problems faced by today's young scientists.
However, Clear Labs applies similar technology used in human clinical trials, but for food products, to find these bad batches.
Similarly, whereas Juul Labs applies strict age-gating when selling its products through its website, many of the accused products appear to be sold with little or no real age-verification processes.
For antigen retrieval, the slides were microwaved twice for 10 minutes in 10 mM Citrate buffer pH 6, and then washed three times 5 minutes in PBS 0.1 M. Blocking was performed with 10% normal goat serum in 2× casein (Vector Labs) applied for 20 minutes at room temperature in a humid chamber.
Additionally, the feasibility of continuous processing was successfully demonstrated on the lab-scale applying a continuously stirred-tank reactor.
Observations from the many E-lab exercises applying both kinds of data underpin the assertion that, 'in practice, qualitative and quantitative are not conducted in differing research paradigms' [25, p.244].
Researchers tend to take a discovery from the lab and apply it to patients; the reverse trip is more and more uncommon.
