In week 2, a few LAs came into the pedagogy class with some constructivist or student ideas language already; about 4% of LA responses demonstrated some ideas about constructing knowledge, while 6% of LA responses indicated that students have pre-existing ideas.
We felt that this was appropriate because while the essential pedagogical principles are distinguishable, they are not mutually exclusive, and we found that LA responses could include some aspects of one or all of the principles.
We note that LA responses could occasionally be double-coded with these subcodes if one part of their response indicated that they were thinking about student ideas as obstacles, while in another section of their response, they demonstrated thinking about student ideas as resources.
As demonstrated in Figure 1, LA caused [Ca2+]i rise in HM cells and the amplitude of the LA responses was dose dependent (Figure 1A and B) with maximal response observed at 30 60 µM.
We tested the role of hsp90β1 in the LA responses by downregulation of hsp90β1 expression with a well-known hsp90 chaperone inhibitor, 17- dimethylaminoethylamino -17-demethoxygeldanamycin (17- dimethylaminoethylamino -17-demethoxygeldanamycin
Downregulation of hsp90β1, which has been previously shown to be essential for polyunsaturated fatty acid-induced mitochondrial Ca2+ efflux, significantly diminished LA-responsive mitochondrial Ca2+ efflux and the coupled peroxynitrite generation, implicating a critical role of hsp90β1 in the LA responses.
Bloom, DD, St Clair, EW, Pisetsky, DS, and Clarke, SH. "The anti-La response of a single MRL/Mp-lpr/lpr mouse: specificity for DNA and VH gene usage". Eur J Immunol 24, no.
In absence of Mecp2, the minimal Cortico-LA response was indeed significantly lower than in WT mice (Fig. S3).
And, if accidentally induced, an anti-La response should not become life threatening as anti-La antibodies are assumed to be non-pathogenic [35] and can even occur in healthy individuals [36].
