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In more advanced lesions, called LA lesions, myelin is completely lost (Fig. 2a, c and d).
In LA lesions, where tissue loss is extensive, we found a general loss of mitochondria (including porin reactive elements) except within phagocytic macrophages (Fig. 5ccc hhh).
Staining for mitochondrial proteins revealed a profound loss of COX-I (Fig. 2e and g) and COX-IV of complex IV (Fig. 2i) in EA and LA lesion areas, while a general reduction of mitochondrial density, visualized also by reduction of porin immunoreactivity, was mainly seen in LA lesions where vacuolation of tissue is particularly apparent.
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Before intervention, the lesion vessel size of deep injury group was smaller than that of the nondeep injury group (p <0.05 for average VA and PA), whereas average lesion LA, lesion length, and reference vessel size did not differ.
Se observaron mediante células positivas LC3 aumentadas en la dermis profunda de las lesions por quemadure la tíncion inmunohistoquímica.
The following morphometric indexes were calculated by mean value of 5 consecutive cross sections of each identified lesion at the site of its maximal extent: maximal intimal thickness (IT), maximal intimal to media thickness ratio (IMT ratio), cross-sectional lesion area (LA), and lesion to intact wall area ratio (LA ratio).
In patients with persistent AF (n = 11), additional isolation of the posterior LA (box lesion) and the placement of a mitral isthmus line were performed.
Conclusion: These data show that in Fe-overload conditions, progressive Fe accumulation occurs in fiber-laden macrophages within LA-induced lesions.
However, the lack of persistence in LA-induced inflammatory gene expression despite Fe accumulation in SHHF rats is not consistent with its involvement in LA-induced hyperplastic lesions.
Ablation lines were also deployed along the LA roof and LA posterior wall joining contralateral encircling lesions, and along the mitral isthmus.
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