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Western blot analysis did not reveal differences in LA expression levels of pro-caspase-3 and pro-caspase-9 between paced and control pigs, nor signs of activation of these key apoptotic enzymes in any of LA samples studied.
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The relative abundance of each band was quantified via the Bio-profile Bio-1 D application Vilber-Lourmat, Marinee la Vallee, France), and the expression levels were normalized to β-actin.
The relative abundance of each band was quantified by using the Bio-profile Bio-1D application (Vilber-Lourmat, Marine la Vallee, France), and the expression levels were normalized to β-actin.
The gels were scanned using the 'DNA Analyzer' system (Vilber Lourmat, Marne-la-Valle, France) and epimerase expression levels were estimated from the intensity of the amplified GLCE DNA fragment normalised against the intensity of GAPDH (TotalLab programme, Nonlinear Dynamics, Newcastle upon Tyne, UK).
However, expression levels of recombinantly expressed laccases are often low.
Full-genome mRNA expression levels were analyzed using the Applied Biosystems expression array system (Foster City, LA, USA).
They analyzed gene expression levels to come up with several enzymes that could be involved in tartaric acid production.
Gene expression levels were normalized per chip.
Expression levels were determined by qPCR.
All expression levels normalized against GAPDH.
Colors indicate expression levels of DEGs.
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