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In addition, the trans-synaptic tracer labelling result in this study also indicated the SLSC were not retrogradely targeted by the LA cells.
The cell viability test conducted with these fluids on He La cells was promising (not included).
GR-ir was observed at the nucleus of principal and inhibitory LA cells (Fig. 2A and 2E) but also in small dendrites and apparent spines.
The ability of generating intrinsic membrane potential oscillations is hypothesized to endow LA cells with the capacity to behave like unitary oscillators that can exhibit resonant behavior.
Furthermore, DDP treatment could lead to the upregulated expression of miR-224 in DDP-resistant LA cells but have no effects on its expression in parental LA cells.
We have observed a significantly higher level of expression of miR-200 family in LA cells compared with ET (unpublished observation), but increased MYB expression in the LA cells that overexpress all miR-200 family members.
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Lebret et al (2007) showed that CAFs enhanced migration by promoting EMT of PMC42-LA cells.
PMC42-LA cells undergo a marked EGF-induced EMT [ 3, 42, 43].
Wounded control PMC42-LA cells were accompanied by very little vimentin upregulation and incomplete wound closure over 48 hours, similar to PMC42-LA cells cultured in NMF-conditioned medium.
No significant difference was detected at 24 hours, but at 3 days numbers of PMC42-LA cells in CAF-conditioned medium were considerably greater than numbers of PMC42-LA cells cultured in NMF-conditioned medium.
In the presence of EGF, PMC42-LA cells in two-dimensional culture undergo an EMT [ 25, 26].
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