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About 10 g of the grounded leaves of P. europaea and P. auriculata plants were soaked separately in 1 l of three different solvents (water, methanol, and acetone), and each of the extracts was placed in a shaker device at 100 rounds per minute for 72 h at room temperature and then stored in a refrigerator for 4 days.
The column was externally marked to indicate three compartments: an upper compartment (U) 12 cm in height, a middle compartment (M) of 10 cm and a lower compartment (L) of three cm in height.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com