Sentence examples for l buffer from inspiring English sources
E. coli cells were cultivated in terrific broth (TB) medium (12 g tryptone, 24 g yeast, 4 g glycerol in 1 L buffer autoclaved separately).
One-hundred fifty grams of frozen engorged ticks were homogenised in 1 l buffer A (20 mM Tris-HCl, pH 8.0, 1 mM CaCl2, 1 mM benzamidine).
The column was then washed with 500 μl l Buffer AW1 by centrifugation at 8000 rpm for 1' and with 500 μl l Buffer AW2 by centrifugation at 14000 rpm for 3'.
The resin was resuspended in 500 l buffer containing 25 mM Tris, pH 8.0, and 150 mM NaCl.
The pure protein was dialyzed against 5 l buffer A overnight, concentrated to 8 mg/ml and analyzed by SDS-PAGE and subsequent Coomassie staining.
For the neuropathological evaluation, the contralateral half-brain is used, immersed in 2.0 l buffered, 10% formalin solution, at room temperature, over 7 10 days.
To render the protein Ca2+-free, an EDTA solution was added to the protein samples to make the final concentration of 5 mM followed by dialysis against a 1-L buffer containing 50 mM Tris, pH 7.5 and 1 mM EDTA overnight at 4°C.
2.5 g of powdered cells were re-suspended in 10 mL of ChIP-L buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl, pH 8.1) containing a cocktail of fungal protease inhibitors (Sigma) and mixed by inversion for 30 min on ice.
Buffer L was Buffer E plus 50 mM EDTA.
The cells were lysed in the L-buffer (20 mM Tris-HCl, pH 8.0, 0.5% Triton X-100, 0.4 mM phenylmethylsulfonyl fluoride) by sonication, and then NaCl and imidazole were added to final concentrations of 0.5 and 0.02 M, respectively.
