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The overall goal of the PEAK program was to promote physical therapists' integration of research evidence into clinical decision-making at the individual (i.e., EBP) and organizational (i.e., KT) level.
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(F and H ) Quantification of Bub1 and BubR1 KT levels, respectively, in cells treated as in panel E and G.
In addition, the timing of the masculinization seemed to be related to the steroid concentration: the later this process occurred, the higher the 11-KT level and the lower the E2 level.
In females, 11-KT levels were low and E2 levels were high, whereas the opposite was found in all males.
In all masculinized females, 11-KT levels were significantly higher than those in females, but lower than in all males.
High 11-kt levels in male fish have been correlated with behavioural dominance (Borg 1994) and increased reproductive success (Coe et al. 2008).
The sonicates were centrifuged at 20,000 g for 20 min at 4°C and the supernatants stored at −70°C prior to the analysis of E2 and 11-KT levels.
Negative correlations were demonstrated between the AVT and 11-KT levels in naïve males (r = −0.50; P<0.01), older females (r = −0.54; P<0.001) and older masculinized females (r = −0.62; P<0.001).
However, in black molly, 11-KT levels were considerably lower in masculinized females than in males, despite the fact that both have fully developed gonopodia.
However, in this study, outbred exposed males had high 11-kt levels and increased reproductive success, corresponding with an increase in paternity share of offspring.
Although disruption to plasma 11-kt levels is consistent with the mode of action of clotrimazole (aromatase inhibition), it is unclear why this response would differ between inbred and outbred fish.
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