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The location and timing data for known replication origins were extracted from oriDB (www.oridb.org and [48]).
The possibility that c-Myc may exert its effect upon DNA replication though alteration of chromatin structure is intriguing in light of the role of c-Myc in recruiting histone acetylase complexes [42] and its direct binding to known replication origins [1].
The I2 cluster, which peaks during S phase, contains known replication fork proteins ("DNA replication," "DNA polymerase," and "RF-C").
It was reported that MCM8 may play a role in elongation and was strongly suggested that MCM8 functions with other known replication proteins in processes which accompany initiation of DNA replication.
We address this issue in our current study by analyzing nucleotide composition in genome regions between known replication origins, with an aim to reveal any adenine to guanine deamination gradients.
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As expected, the known replication-independent histone variant H2A.Z continued to incorporate heavy arginine at the same rate after the thymidine block as before.
We analyzed the expression of polyadenylated H2B1D (H2B1Dpandall all H2B1D transcripts (H2B1D), as well as the expression of a known replication-dependent histone variant (H3.1), a known replication-independent histone variant (H3.3), and a histone H2B isoform with no polyadenylation signal (H2B1C) as controls.
SNPs in the vicinity of the IL-5 gene were identified as being associated with clinical disease in a gene-centric study of CAD [11], but as far as we know, replication of this locus has not been reported.
Similar to bacteria, archaea contain circular chromosomes and initiate chromosome replication at specific sites known as replication origins (Robinson and Bell 2005).
However, the capability of VACV-infected S2 cells to replicate exogenous DNA, which is dependent on all known viral replication proteins [43], can be tested by transfection of plasmids.
Replication of a eukaryotic genome is initiated from multiple specific sites along the chromosomes known as replication origins during the S phase of the mitotic cell cycle.
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